게시판 최근논문

[하이라이트 논문] 한국광학회지 Vol. 34 No.2 (2023 April)

사무국 hit 680 date 2023-04-25

적색 및 적외선 빛을 이용한 Photobiomodulation: 각막상피세포에 대한 효과와 상처 치유에 관한 연구
Photobiomodulation Mediated by Red and Infrared Light: A Study of Its Effectiveness on Corneal Epithelial Cells and Wound Healing

안선희1ㆍ안재성1ㆍ이병일2†

 

한국광학회지  Vol. 34 No.2 (2023 April) pp. 45-52
DOI: https://doi.org/10.3807/KJOP.2023.34.2.045

 


 



Fig. 1  Representative picture of the experimental setup and emission spectra of light-emitting diodes (LEDs). (a) LEDs were irradiated to the 24-well plate from the top and a single-well-shaped mask was fixed on the top surface of the well plate to prevent LED light from being irradiated to adjacent wells. The power of LED light transmitted through the mask was measured with a photodiode sensor to derive the amount of light energy irradiated to the sample. (b) Normalized spectra of LED light sources.


 

Keywords: 각막상피세포, 저출력광치료, 광생물조절, 상처치료
OCIS codes: (170.1530) Cell analysis; (350.5130) Photochemistry

 


 

초록
본 연구에서는 다양한 파장의 저출력 light-emitting diode (LED)를 이용한 photobiomodulation (PBM)이 각막 상처 치유에 미치는 영향을 분석하였다. 각막상피세포에 623 nm에서 940 nm 범위의 파장의 LED를 조사한 결과, 유의미한 세포독성 영향을 미치지 않는 것을 확인하였다. PBM의 세포이동 촉진 효과를 세포 이동능 평가 시험을 통해 분석한 결과 623 nm 파장의 광조사에 의한 PBM이 세포이동을 크게 증가시키고 상처 치유를 촉진하는 것으로 나타났다. 또한, 세포이동 및 상처 치유와 관련된 유전자의 발현을 분석한 결과, 623 nm 파장의 광조사에 의한 PBM이 세포 증식과 세포 외 기질 분해를 촉진하는 것으로 알려진 FGF-1과 MMP2 유전자의 발현을 상향 조절한다는 사실을 발견했다. 이러한 연구 결과는 특정 파장, 특히 623 nm 파장의 저출력 빛을 이용한 PBM이 각막 손상 치료에 활용될 수 있는 가능성을 시사한다.
 
Abstract
In this study, we have investigated the effect of photobiomodulation (PBM) on corneal wound healing, using a low-power light-emitting diode (LED) at different wavelengths. We found that LEDs with wavelengths ranging from 623 to 940 nm had no significant cytotoxic effects on corneal epithelial cells. The effect of PBM on promoting cell migration was analyzed by scratch assay, and it was found that PBM at 623 nm significantly increased cell migration and promoted wound healing. Furthermore, the expression of genes related to cell migration and wound healing was analyzed, and it was found that PBM at 623 nm upregulated the expression of the genes FGF-1 and MMP2, which are known to promote cell proliferation and extracellular matrix degradation. These findings suggest that PBM with low-powered light at specific wavelengths, particularly 623 nm, could be utilized to treat corneal injury.